Gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells.
- Author:
Qingliu HU
1
;
Yingjie PIAO
;
Fei ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Bone Marrow Cells; Gene Expression Profiling; Humans; Mesoderm; cytology; Stem Cells; Tendons; cytology
- From: Chinese Medical Journal 2003;116(8):1270-1272
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells.
METHODSTotal RNA extracted from human bone marrow derived mesenchymal stem cells and tendon cells underwent reverse transcription, and the products were labeled with alpha-(32)P dCTP. The cDNA probes of total RNA were hybridized to cDNA microarray with 1176 genes, and then the signals were analyzed by Atlas Image analysis software Version 1.01a.
RESULTSFifteen genes associated with cell proliferation and signal transduction were up-regulated, and one gene that takes part in cell-to-cell adhesion was down-regulated in tendon cells.
CONCLUSIONThe 15 up-regulated and one down-regulated genes may be beneficial to the orientational differentiation of mesenchymal stem cells into tendon cells.
