Apoptosis in Raji cell line induced by influenza A virus.
- Author:
Hong LI
1
;
Liying XIAO
;
Hualin LI
;
Wanyi LI
;
Zhonghua JIANG
;
Lin ZHANG
;
Mingyuan LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; physiology; Humans; Influenza A virus; physiology; Tumor Cells, Cultured
- From: Chinese Medical Journal 2003;116(9):1321-1324
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the apoptotic effects of influenza A virus on the Raji cell line.
METHODSCultured Raji cells were infected with influenza A virus at a multiplicity of infection (m.o.i) of 20 and the effects of apoptosis were detected at different time points post infection using the following methods: electron microscope, DNA agarose gel electrophoresis, PI stained flow cytometry (FCM) and Annexin-V FITC/PI stained FCM.
RESULTSRaji cells infected with influenza A virus showed changes of morphology apoptosis, DNA agarose electrophoresis also demonstrated a ladder-like pattern of DNA fragments in a time-dependent manner. PI stained FCM showed "apoptosis peak" and FITC/PI stained FCM showed apoptotic cells. Quantitative analysis indicated that the percentage of apoptotic Raji cells increased after infection, and cycloheximide (CHX), an eukaryotic transcription inhibitor, could effectively inhibit the apoptotic effects of influenza A virus in vitro.
CONCLUSIONSInfluenza A virus can induce apoptosis in Raji cell line suggesting that it may lead to a potential method for tumor therapy.