Effects of magnesium sulfate on neuron apoptosis and expression of caspase-3, bax and bcl-2 after cerebral ischemia-reperfusion injury.
- Author:
Haiyan ZHOU
1
;
Yuefeng MA
;
Yifeng ZHOU
;
Ziming LIU
;
Kai WANG
;
Gang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 3; Caspases; analysis; Cerebrovascular Disorders; metabolism; pathology; Gerbillinae; Magnesium Sulfate; pharmacology; Neurons; cytology; drug effects; Proto-Oncogene Proteins; analysis; Proto-Oncogene Proteins c-bcl-2; analysis; Random Allocation; Reperfusion Injury; metabolism; pathology; bcl-2-Associated X Protein
- From: Chinese Medical Journal 2003;116(10):1532-1534
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of magnesium sulfate on neuron apoptosis and the expressions of caspase-3, bax and bcl-2 after cerebral ischemia-reperfusion injury.
METHODSThirty-six gerbils were randomly divided into three groups: Sham-operation group (So, n = 12), ischemia-reperfusion group (I-R, n = 12) and magnesium sulfate group (Ms, n = 12). The neuron apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-fluorescence nick end labeling (TUNEL stain), and the protein expressions of caspase-3, bax and bcl-2 were detected by immunohistochemisty stain.
RESULTSApoptotic neurons and the expressions of caspase-3 and bax were significantly increased in I-R and Ms groups, compared with those in So group. Apoptotic neurons and the expressions of bax and caspase-3 in Ms group were significantly less than those in I-R group. There was no significant difference in the expression of bcl-2 among three groups.
CONCLUSIONSMagnesium sulfate decreases neuron apoptosis after cerebral ischemia-reperfusion injury, which may be related with the suppressed expressions of caspase-3 and bax.
