Unique GGT --> GTT mutation at K-ras codon 12 in six human pancreatic cancer cell lines from Chinese patients.
- Author:
Shuanzeng WEI
1
;
Tonghua LIU
;
Hongrui LIU
;
Jie GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; Asian Continental Ancestry Group; Cell Line, Tumor; China; Codon; Genes, ras; genetics; Humans; Mutation; Pancreatic Neoplasms; genetics
- From: Chinese Medical Journal 2003;116(10):1585-1587
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients.
METHODSAll six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing.
RESULTSAll 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13.
CONCLUSIONThe unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.
