Effect of Different Hemapheresis Procedures on Components of Hematopoietic Stem Cells Collected from Healthy Donors.

Chun-Hong SUN; Jian-Na YAO; Fang LIU; Xiao-Juan WANG; Juan-Juan MA; Mi-Mi CUI; Mei ZHANG; Xiao-Ning WANG

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Effect of Different Hemapheresis Procedures on Components of Hematopoietic Stem Cells Collected from Healthy Donors.

Author: Chun-Hong SUN ¹ ; Jian-Na YAO ¹ ; Fang LIU ¹ ; Xiao-Juan WANG ¹ ; Juan-Juan MA ¹ ; Mi-Mi CUI ¹ ; Mei ZHANG ² ; Xiao-Ning WANG ³
Author Information

1. Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
2. Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail: zhangmeimeidmail@163.com.
3. Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail: wangxn99@163.com.
Publication Type:Journal Article
From: Journal of Experimental Hematology 2016;24(6):1869-1872
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effects of different hemapheresis procedures on the components of hematopoietic stem cells(HSCs) collected from helathy donors.
METHODStwelve donors were underwent stem cell collection from January 2015 to August 2016, and the stem cells were randomly colleted by AutoPBSC procedure of COBE spectra and MNC procedure of the Spectra Optia blood cell separator, the mononuclear cells, CD34cells, granulocytes, lymphocytes and platelets in the collections were compared.
RESULTSThe circulating blood volume, the acquisition time and dosage of anticoagulants were not significantly different between two procedures. The volume and the mononuclear cell count collected by AutoPBSC procedure were lower than those by the MNC procedure, while the CD34cell count by AutoPBSC procedure was higher than that by the MNC procedure. More lymphocytes and platelets were collected by AutoPBSC procedure as compared with that by the MNC procedure (P<0.05).
CONCLUSIONCompared with MNC procedure of the Spectra Optia blood cell separator, the number of collected stem cells, lymphocytes and platelets are higher by using AutoPBSC procedure of the COBE spectra blood cell separator.