Antimicrobial resistance and molecular epidemiologic characteristics of Stenotrophomonas maltophilia isolated from clinical specimens.
- Author:
Sung Yong SEOL
1
;
Kyoung Soo JANG
;
Oung Gi JEONG
;
Eung Rae CHO
;
Neung Hee KIM
;
Hak Sun YU
;
Yoo Chul LEE
;
Dong Taek CHO
Author Information
1. Department of Microbiology, School of Medicine, Kyungpook National University, Taegu, South Korea.
- Publication Type:Original Article
- MeSH:
Amikacin;
Aminoglycosides;
Ampicillin;
Anti-Bacterial Agents;
beta-Galactosidase;
Carbenicillin;
Cefotaxime;
Cefoxitin;
Ceftazidime;
Chloramphenicol;
Ciprofloxacin;
Consensus Sequence;
Daegu;
Gentamicins;
Hospitals, University;
Humans;
Inpatients;
Microbial Sensitivity Tests;
Minocycline;
Nalidixic Acid;
Polymerase Chain Reaction;
Stenotrophomonas maltophilia*;
Stenotrophomonas*;
Sulfisomidine;
Tobramycin;
Trimethoprim;
Wounds and Injuries
- From:Journal of the Korean Society for Microbiology
2000;35(3):239-250
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Sixty-eight clinical isolates of Stenotrophomonas maltophilia from inpatients of 2 university hospitals in Taegu were epidemiologically analyzed by using the minimum inhibitory concentrations of 25 antimicrobial drugs, biochemical reaction, pulsed-field gel elctropgoresis (PFGE), and PCR with enterobacterial repetitive intergenic consensus sequences as primer (ERIC-PCR). 1. All the strains were susceptible to minocycline. More than 57% were susceptible to sulfisomidine (Su), ciprofloxacin (Ci), Ofloploxacin (Of), nalidixic acid (Na), and chloramphenicol (Cm), and 19apprx35% to ceftazidime (Cd), trimethoprim (Tp), Ticacillin-clavulanic acid, and cefoperazone-sulbactam. Most isolates were resistant to beta-lactam antibiotics such as ampicillin (Ap), carbenicillin (Cb), cefotaxim (Ct), cefoxitin (Cx), and aminoglycosides including gentamicin (Gm), tobramycin (Tb), amikacin (Ak). 2. All the isolates were multiply resistant of 5 to 17 drugs and showed 40 different resistance pattern types. 3. All the strains showed very similar biochemical reactions except beta-galactosidase and nitrate reduction test. Fourteen strains selected randomly were classified 10 different pattern type by PFGE and ERIC-PCR. These two methods showed identical result. Four strains isolated from wound in 1994 showed similar MIC pattern and identical API 20NE profile, PFGE, and ERIC-PCR pattern indicating episodes of cross-infection among patients. These results indicate that PFGE or ERIC-PCR profile has comparable discriminatory power for epidemiological typing of S. maltophilia.
