Effects of silicon dioxide on expression of alpha-smooth muscle actin in human lung fibroblasts.
- Author:
Yong-bin HU
1
;
Qing-fu ZENG
;
Jin-wu PENG
;
Ling CHU
;
Xiang LI
;
Jin-sheng WANG
;
Hai-ying JIANG
;
Ji-fang WEN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; biosynthesis; genetics; Animals; Cells, Cultured; Fibroblasts; drug effects; metabolism; Lung; cytology; metabolism; Macrophages, Peritoneal; drug effects; Rats; Rats, Sprague-Dawley; Silicon Dioxide; pharmacology; Silicosis; metabolism; pathology; Transforming Growth Factor beta1; pharmacology
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(9):523-525
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of SiO(2) on the expression of alpha-smooth muscle actin (alpha-SMA) in human lung fibroblasts in vitro and vivo.
METHODSThe experimental group comprised 32 rats while 32 rats were included in the control. In vivo, the expression of alpha-SMA in lung tissues of rats exposed to SiO(2), the supernate of RAW264.7 cells, SiO(2) and the growth factor beta(1) (TGF-beta(1)) were investigated, respectively.
RESULTS(1) alpha-SMA positive myofibroblasts appeared in the lung tissues of the 28th day groups exposed to SiO(2). (2) The expression of alpha-SMA in HLF-02 cells was unregulated by TGF-beta(1) and supernate of RAW264.7 cells exposed to SiO(2). (3) The expression of alpha-SMA in HLF-02 cells was not induced by SiO(2).
CONCLUSIONMyofibroblasts related to silicosis, and the appearance of myofibroblasts (in vitro) are independent on direct stimulation by SiO(2), but related to the mediator (TGF-beta(1)) secreted by SiO(2) stimulated macrophages.
