Relationship between single nucleotide polymorphisms of NRAMP1 gene and susceptibility to pulmonary tuberculosis in workers exposed to silica dusts.
- Author:
Ya-bin QU
1
;
Yun-xia TANG
;
Zhong-bin ZHANG
;
Ren ZHU
;
Jing LIU
;
Shou-yong GU
;
Guo-liang LU
;
Zhao-lin XIA
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Alleles; Case-Control Studies; Cation Transport Proteins; genetics; Genetic Predisposition to Disease; Humans; Middle Aged; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Silicosis; complications; Tuberculosis, Pulmonary; complications; genetics
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(9):531-533
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between polymorphisms of natural resistance-associated macrophage protein 1 (NRAMP1) gene and genetic susceptibility of pulmonary tuberculosis (PTB) in workers exposed to silica dusts.
METHODSA 1:2 case control study of 61 male workers with PTB (50 silicosis patients and 11 unsilicosis workers) as the case group and 122 male PTB-free workers (100 silicosis patients and 22 unsilicosis workers) as the control group was conducted with the frequency matched for age of +/- 5 years, the job, the silica exposure, and the condition of cigarette smoking and alcohol drinking. The polymerase chain reaction-restrained fragment length polymorphism technique (PCR-RFLP) was used to detect the single nucleotide polymorphisms (SNPs) of NRAMP1 INT4 and D543N.
RESULTSThere was a 2.73 times (95% CI: 1.32 approximately 5.64) increased risk of silicosis for individuals with C allele of NRAMP1 INT4 compared with individuals carrying homozygote (G/G), while SNPs of NRAMP1 D543N was not associated with PTB (P > 0.05).
CONCLUSIONThe G > C mutation of intron 4 of NRAMP1 gene might be a susceptible factor of silica for the workers exposed to PTB.
