Differential Proteomics Reveals the Potential Injury Mechanism Induced by Heavy Ion Radiation in Mice Ovaries.

Author: Yu Xuan HE ¹ ; Hong ZHANG ² ; Hong Yan LI ² ; Yong ZHANG ¹ ; Qi Peng JIA ¹ ; Zong Shuai LI ¹ ; Xing Xu ZHAO ¹
Author Information

1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
2. Department of Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China
Publication Type:Letter
MeSH: Animals; Biomarkers; Carrier Proteins; genetics; metabolism; Electrophoresis, Gel, Two-Dimensional; Female; Gene Expression; Heavy Ion Radiotherapy; adverse effects; Mice; Ovary; radiation effects; Proteomics; Random Allocation; Ubiquitin Thiolesterase; genetics; metabolism
From: Biomedical and Environmental Sciences 2017;30(4):301-307
CountryChina
Language:English
Abstract: In the present study, we used a proteomics approach based on a two-dimensional electrophoresis (2-DE) reference map to investigate protein expression in the ovarian tissues of pubertal Swiss-Webster mice subjected to carbon ion radiation (CIR). Among the identified proteins, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is associated with the cell cycle[1] and that it influences proliferation in ovarian tissues. We analyzed the expression of UCH-L1 and the proliferation marker proliferation cell nuclear antigen (PCNA) following CIR using immunoblotting and immunofluorescence. The proteomics and biochemical results provide insight into the underlying mechanisms of CIR toxicity in ovarian tissues.