Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China.

Yin Ping Dong; Wei Wang; Tao Jiang; Jin XU; Chun Hui Han; Shao Fei Yan; Séamus Fanning; Ying LI; Xiao Chen MA; Di Zhang; Yao Zhao; Biao Zeng; Feng Qin LI

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Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China.

Author: Yin Ping DONG ¹ ; Wei WANG ¹ ; Tao JIANG ¹ ; Jin XU ¹ ; Chun Hui HAN ¹ ; Shao Fei YAN ¹ ; Séamus FANNING ² ; Ying LI ³ ; Xiao Chen MA ⁴ ; Di ZHANG ³ ; Yao ZHAO ⁴ ; Biao ZENG ⁴ ; Feng Qin LI ¹
Author Information

1. Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100021, China.
2. Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100021, China
3. Children's Hospital Affiliated to Capital Institute of Pediatrics, Beijing 100020, China.
4. Beijing Centers for Disease Control and Prevention, Beijing 100013, China.
Publication Type:Case Reports
Keywords: China; Clostridium botulinum; Infant botulism; Powdered infant formula
MeSH: Animals; Beijing; epidemiology; Botulinum Toxins; isolation & purification; toxicity; Botulism; diagnosis; epidemiology; Clostridium botulinum; isolation & purification; Gastrointestinal Tract; microbiology; Humans; Infant; Mice; Toxicity Tests
From: Biomedical and Environmental Sciences 2017;30(6):460-464
CountryChina
Language:English
Abstract: Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula (PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins (BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis (PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores.