First Report in China on the Identification and Drug Sensitivity of Mycobacterium elephantis Isolated from the Milk of a Cow with Mastitis.

Ling Yun JI; Dong Lei XU; Shu Peng Yin; Hai Can Liu; Gui Lian LI; Yi Jiang; Jian Hao Wei; Hao Zeng; Yong Liang Lou; Jian Xin Lyu; Kang Lin Wan

Return

First Report in China on the Identification and Drug Sensitivity of Mycobacterium elephantis Isolated from the Milk of a Cow with Mastitis.

Author: Ling Yun JI ¹ ; Dong Lei XU ² ; Shu Peng YIN ³ ; Hai Can LIU ² ; Gui Lian LI ² ; Yi JIANG ² ; Jian Hao WEI ⁴ ; Hao ZENG ⁵ ; Yong Liang LOU ³ ; Jian Xin LYU ³ ; Kang Lin WAN ⁶ ;
Author Information

1. Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China.
2. State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, China.
3. Key Laboratory of Laboratory Medicine, Ministry of Education, College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
4. Department of Clinical Laboratory, Shanghai Public Health Clinical Center, Affiliated to Fudan University, Shanghai 201508, China.
5. Department of Spine Surgery, The Xiangya Hospital of Central South University, Changsha 410008, Hunan, China.
6. State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention Beijing 102206, China
Publication Type:Journal Article
Keywords: Cow mastitis; Milk; Mycobacterium elephantis; Non-tuberculosis mycobacterium
MeSH: Animals; Anti-Bacterial Agents; pharmacology; Cattle; China; Drug Resistance, Bacterial; Female; Mastitis, Bovine; epidemiology; microbiology; Milk; microbiology; Mycobacterium; drug effects; genetics; isolation & purification; Mycobacterium Infections; epidemiology; microbiology; veterinary; Phylogeny; Polymerase Chain Reaction
From: Biomedical and Environmental Sciences 2017;30(7):501-507
CountryChina
Language:English
Abstract:
OBJECTIVEIn this study, milk from a cow with mastitis was analyzed to determine the presence of mycobacterial infection. Milk quality and security problems pertaining to the safe consumption of dairy products were also discussed in this study.
METHODSMilk was preprocessed with 4% NaOH. Then, mycobacteria were isolated from the milk sample on L-J medium. The isolate was identified using multiple loci Polymerase Chain Reaction (PCR) and multi-locus sequence analysis with 16S rRNA, sodA, hsp65, and ITS genes. The drug sensitivity of the isolate to 27 antibiotics was tested through alamar blue assay.
RESULTSSmooth, moist, pale yellow colonies appeared on the L-J medium within a week after inoculation. Based on the results of multiple loci PCR analysis, the isolate was preliminarily identified as non-tuberculous mycobacteria. The 16S rRNA, SodA, hsp65, and ITS gene sequences of the isolate exhibited 99%, 99%, 99%, and 100% similarities, respectively, with those of the published reference strains of Mycobacterium elephantis (M. elephantis). The drug sensitivity results showed that the strain is resistant to isoniazid, p-aminosalicylic acid, and trimesulf but is sensitive to ofloxacin, rifampicin, amikacin, capreomycin, moxifloxacin, kanamycin, levofloxacin, cycloserine, ethambutol, streptomycin, tobramycin, rifabutin, ciprofloxacin, linezolid, cefoxitin, clarithromycin, and minocycline.
CONCLUSIONTo the best of our knowledge, this study is initially to report the isolation of M. elephantis from the milk of a cow with mastitis in China.