A Five-year Surveillance of Carbapenemase-producing Klebsiella pneumoniae in a Pediatric Hospital in China Reveals Increased Predominance of NDM-1.

Fang Dong; Jie LU; Yan Wang; Jin Shi; Jing Hui Zhen; Ping Chu; Yang Zhen; Shu Jing Han; Yong Li Guo; Wen Qi Song

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A Five-year Surveillance of Carbapenemase-producing Klebsiella pneumoniae in a Pediatric Hospital in China Reveals Increased Predominance of NDM-1.

Author: Fang DONG ^{1
,

2} ; Jie LU ³ ; Yan WANG ¹ ; Jin SHI ³ ; Jing Hui ZHEN ¹ ; Ping CHU ³ ; Yang ZHEN ¹ ; Shu Jing HAN ³ ; Yong Li GUO ³ ; Wen Qi SONG ¹ ;
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1. Department of Laboratory Medicine, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China
2. Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
3. Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China
Publication Type:Journal Article
Keywords: Carbapenemases; Klebsiella pneumoniae; Microbial drug resistance
MeSH: Anti-Bacterial Agents; pharmacology; Bacterial Proteins; genetics; metabolism; Child; China; epidemiology; Drug Resistance; Gene Expression Regulation, Bacterial; physiology; Gene Expression Regulation, Enzymologic; physiology; Hospitals, Pediatric; Humans; Klebsiella Infections; epidemiology; microbiology; Klebsiella pneumoniae; drug effects; enzymology; genetics; Microbial Sensitivity Tests; Population Surveillance; Time Factors; beta-Lactamases; genetics; metabolism
From: Biomedical and Environmental Sciences 2017;30(8):562-569
CountryChina
Language:English
Abstract:
OBJECTIVETo characterize carbapenem (CPM)-non-susceptible Klebsiella pneumoniae (K. pneumoniae) and carbape-nemase produced by these strains isolated from Beijing Children's Hospital based on a five-year surveillance.
METHODSThe Minimal Inhibition Concentration values for 15 antibiotics were assessed using the Phonix100 compact system. PCR amplification and DNA sequencing were used to detect genes encoding carbapenemases. WHONET 5.6 was finally used for resistance analysis.
RESULTSIn total, 179 strains of CPM-non-susceptible K. pneumoniae were isolated from January, 2010 to December, 2014. The rates of non-susceptible to imipenem and meropenem were 95.0% and 95.6%, respectively. In the 179 strains, 95 (53.1%) strains carried the blaIMP gene, and IMP-4 and IMP-8 were detected in 92 (96.8%) and 3 (3.2%) IMP-producing isolates, respectively. 65 (36.3%) strains carried the blaNDM-1 gene. 6 (3.4%) strains carried the blaKPC gene, and KPC-2 were detected in 6 KPC-producing isolates. In addition, New Delhi-Metallo-1 (NDM-1) producing isolates increased from 7.1% to 63.0% in five years and IMP-4 producing isolates decreased from 75.0% to 28.3%.
CONCLUSIONHigh frequencies of multiple resistances to antibiotics were observed in the CPM-non-susceptible K. pneumoniae strains isolated from Beijing Children's Hospital. The production of IMP-4 and NDM-1 metallo-β-lactamases appears to be an important mechanism for CPM-non- susceptible in K. pneumoniae.