Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China.

Wei Wang; Feng Liu; Zulqarnain Baloch; Cun Shan Zhang; Ke MA; Zi Xin Peng; Shao Fei Yan; Yu Jie HU; Xin Gan; Yin Ping Dong; Yao Bai; Feng Qin LI; Xiao Mein Yan; Ai Guo MA; Jin XU

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Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China.

Author: Wei WANG ^{1
,

2} ; Feng LIU ¹ ; Zulqarnain BALOCH ³ ; Cun Shan ZHANG ⁴ ; Ke MA ⁵ ; Zi Xin PENG ⁶ ; Shao Fei YAN ⁶ ; Yu Jie HU ⁶ ; Xin GAN ⁶ ; Yin Ping DONG ⁶ ; Yao BAI ⁶ ; Feng Qin LI ⁶ ; Xiao Mein YAN ⁷ ; Ai Guo MA ⁵ ; Jin XU ⁶
Author Information

1. School of Public Health, Qingdao University, Qingdao 266071, Shandong, China
2. Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
3. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, China.
4. Kuiwen District Center for Disease Control and Prevention, Weifang 261000, Shandong, China.
5. School of Public Health, Qingdao University, Qingdao 266071, Shandong, China.
6. Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
7. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Publication Type:Journal Article
Keywords: Antimicrobial susceptibility; Genetic diversity; Methicillin-resistant Staphylococcus aureus; Pig; Retail food
MeSH: Animals; Anti-Bacterial Agents; pharmacology; China; Food Microbiology; Humans; Methicillin; pharmacology; Methicillin Resistance; Methicillin-Resistant Staphylococcus aureus; genetics; isolation & purification; Nose; microbiology; Swine; microbiology
From: Biomedical and Environmental Sciences 2017;30(8):570-580
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food.
METHODSSeventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing.
RESULTSAll isolated MRSA exhibited multi-drug resistance (MDR). Greater diversity was found in food-associated MRSA (7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA (3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates (40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone (96.4%), and CC9-ST9-t437-MC621 (20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig- and food-associated isolates. Of note, some community- and hospital-associated MRSA strains (t030, t172, t1244, and t4549) were also identified as food-associated isolates.
CONCLUSIONCC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China.