Examine the Correlation between Heat Shock Protein IbpA and Heat Tolerance in Cronobacter sakazakii.

Author: Zhi Jing ZHAO ¹ ; Bin WANG ¹ ; Jing YUAN ² ; Hao Yu LIANG ¹ ; Si Guo DONG ¹ ; Ming ZENG ¹
Author Information

1. National Institutes for Food and Drug Control, Beijing 100050, China.
2. Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100850, China.
Publication Type:Journal Article
MeSH: Bacterial Proteins; genetics; metabolism; Cronobacter sakazakii; genetics; physiology; Gene Expression Regulation, Bacterial; physiology; Genotype; Heat-Shock Proteins; genetics; metabolism; Hot Temperature; Stress, Physiological
From: Biomedical and Environmental Sciences 2017;30(8):606-610
CountryChina
Language:English
Abstract: We used a proteomic approach to identify IbpA in Cronobacter sakazakii (C. sakazaki), which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C. sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 ΔibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 ΔibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coli O157:H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.