Increased PIT1 and PIT2 Expression in Streptozotocin (STZ)-induced Diabetic Mice Contributes to Uptake of iAs(V).

Sha Li YU; Ling Fei XU; Jun Xia WU; Chen Juan Yao; Qiao Yun HU; Chun Xue Zhang; Xin Yuan Zhao; Hai Yan Wei; Xiao Ke Wang; Gang Chen

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Increased PIT1 and PIT2 Expression in Streptozotocin (STZ)-induced Diabetic Mice Contributes to Uptake of iAs(V).

Author: Sha Li YU ¹ ; Ling Fei XU ¹ ; Jun Xia WU ¹ ; Chen Juan YAO ² ; Qiao Yun HU ¹ ; Chun Xue ZHANG ¹ ; Xin Yuan ZHAO ¹ ; Hai Yan WEI ¹ ; Xiao Ke WANG ¹ ; Gang CHEN ¹
Author Information

1. Department of Environmental Health, School of Public Health, Nantong University, Nantong 226019, Jiangsu, China.
2. Department of Molecular Oral Physiology, Institute of Biomedical Sciences, Tokushima University Graduate School, 770-8504, Japan.
Publication Type:Journal Article
Keywords: Pentavalent inorganic arsenic; Phosphate cotransporters; Type I diabetes mellitus; Uptake
MeSH: Animals; Arsenic; pharmacokinetics; Diabetes Mellitus, Experimental; metabolism; Environmental Pollutants; pharmacokinetics; Gene Expression Regulation; physiology; Male; Mice; Mice, Inbred ICR; Sodium-Phosphate Cotransporter Proteins, Type III; genetics; metabolism; Transcription Factor Pit-1; genetics; metabolism
From: Biomedical and Environmental Sciences 2017;30(11):792-801
CountryChina
Language:English
Abstract:
OBJECTIVEThis study aimed to investigate the susceptibility of mice with streptozotocin(STZ)-induced diabetes mellitus (TIDM) to the uptake of pentavalent inorganic arsenic (iAsV) and the possible molecular mechanism.
METHODSTIDM was induced in mice by STZ. TIDM and normal mice were treated with 15.0 mg/kg Na2HAsO4·12H2O by intragastric administration. Then, the concentrations of arsenic in various tissues were measured by atomic fluorescence spectrometry. The gene expression levels of Pit1 and Pit2 were quantified by real-time RT-PCR, and their protein levels were detected by Western blotting in mouse heart, kidney, and liver tissues.
RESULTSThe concentrations of arsenic in STZ-induced TIDM mouse tissues were higher at 2 h after intragastric administration of Na2HAsO4·12H2O. Compared with the levels in normal mice, PIT1 and PIT2, which play a role in the uptake of iAsV, were upregulated in the livers and hearts of TIDM mice. PIT1 but not PIT2 was higher in TIDM mouse kidneys. The upregulation of Pit1 and Pit2 expression could be reversed by insulin treatment.
CONCLUSIONThe increased uptake of iAsV in TIDM mouse tissues may be associated with increased PIT1 and/or PIT2 expression.