Serum Metabonomics of Articular Cartilage Destruction Induced by T-2 Toxin in Wistar Rats.

Lei Zhu; Zhi Jun Zhao; Xiao Bin Ren; Qiang LI; Hua Ding; Zhou Sun; Qing Jun Kao; Li Hua Wang

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Serum Metabonomics of Articular Cartilage Destruction Induced by T-2 Toxin in Wistar Rats.

Author: Lei ZHU ¹ ; Zhi Jun ZHAO ² ; Xiao Bin REN ¹ ; Qiang LI ² ; Hua DING ¹ ; Zhou SUN ¹ ; Qing Jun KAO ¹ ; Li Hua WANG ³
Author Information

1. Division of Infectious Diseases, Hangzhou Center for Disease Control and Prevention, Hangzhou 310021, Zhejiang, China.
2. Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, Qinghai, China.
3. The Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin 150081, Heilongjiang, China.
Publication Type:Journal Article
From: Biomedical and Environmental Sciences 2018;31(1):76-80
CountryChina
Language:English
Abstract: The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction. Thirty healthy male Wistar rats were fed a diet containing T-2 toxin (300 ng/kg chow) for 3 months. Histopathological changes in femorotibial cartilage were characterized in terms of chondrocyte degeneration/necrosis and superficial cartilage defect, and the endogenous metabolite profile of serum was determined by UPLC/Q-TOF MS. Treated rats showed extensive areas of chondrocyte necrosis and superficial cartilage defect in the articular cartilage. In addition, 8 metabolites were found to change significantly in these rats compared to the control group, including lysoPE (18:0/0:0), lysoPC(14:0), lysoPC[18:4 (6Z,9Z,12Z,15Z)], lysoPC[(16:1(9Z)], lysoPC(16:0), L-valine, hippuric acid, and asparaginyl-glycine. These 8 metabolites associated with cartilage injury are mainly involved in phospholipid and amino acid metabolic pathways.