Experimental study on constructing small-caliber artery by tissue engineering approach.
- Author:
Yang LIU
1
;
Yan-zhong ZHANG
;
Jin-jun CHEN
;
De-min YIN
;
Ying CAO
;
Zhi-cheng XU
;
Wei LIU
;
Lei CUI
;
Yi-lin CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arteries; surgery; ultrastructure; Endothelial Cells; ultrastructure; Immunohistochemistry; Mice; Mice, Nude; Microscopy, Electron; Muscle, Smooth, Vascular; ultrastructure; Tissue Engineering; methods
- From: Chinese Journal of Surgery 2003;41(9):679-683
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possibility of constructing small-caliber artery by means of tissue engineering.
METHODSCell-PGA mixtures were made by separately seeding 1 x 10(7) smooth muscle cells and 5 x 10(6) endothelial cells isolated from neonate umbilicus onto PGA scaffold, the cell-PGA constructs were wrapped around a silicone tube before its implantation subcutaneously to nude mice and the mice were sacrificed in 2 and 6 weeks. The tissue engineered artery (TEA) were examined both grossly and immunohistochemically.
RESULTSThe gross appearance of TEA was similar to that of the natural counterparts; histologic and immunohistochemical analyses of the neoformed tissues revealed a typical artery structure, including the presence of EC at the luminal surface and the presence of SMC and collagen in the wall.
CONCLUSIONTEA with histology similar to natural vessel can be constructed by tissue engineering.
