The role of the immunohistochemistry for hMLH1 and hMSH2 with detection of microsatellite instability to identify the kindreds with hereditary nonpolyposis colorectal cancer.
- Author:
Hei-ying JIN
1
;
Long CUI
;
Rong-gui MENG
;
Fei LIU
;
Yu-di YAN
;
Yi-jiang DING
;
Hang YAO
;
Chuan-gang FU
;
De-hong YU
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; Adult; Aged; Carrier Proteins; Colorectal Neoplasms, Hereditary Nonpolyposis; genetics; DNA-Binding Proteins; analysis; Female; Genomic Instability; Humans; Immunohistochemistry; Male; Microsatellite Repeats; Middle Aged; MutL Protein Homolog 1; MutS Homolog 2 Protein; Neoplasm Proteins; analysis; Nuclear Proteins; Proto-Oncogene Proteins; analysis; Sensitivity and Specificity
- From: Chinese Journal of Surgery 2003;41(11):809-811
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the specificity and sensitivity of the immunohistochemistry for hMLH1 and hMSH2 with detection of microsatellite instability (MSI) to identify the kindreds with hereditary nonpolyposis colorectal cancer and to analyse its value in clinical practice.
METHODSpecimens of 16 cases with HNPCC and 16 cases with sporadic colorectal cancer were detected by immunostaining with hMLH1 and hMSH2 and MSI was also detected.
RESULTSThe specificity and sensitivity of the immunohistochemistry for hMLH1 and hMSH2 were 91.7% and 87.5% respectively. The specificity and sensitivity of MSI were 100% and 75.0%. By combining two methods, the specificity and sensitivity were 91.7% and 93.8% respectively.
CONCLUSIONSBy combination of the immunohistochemistry for hMLH1 and hMSH2 and detection of MSI to identify the kindreds with HNPCC, the specificity and sensitivity are improved which is better than to use either of them alone. And it is very easy and cheap that it can be used in clinics.
