Experimental studies on adriamycin alginate-chitosan microcapsules in the treatment of rabbit VX2 carcinoma in the extremity.
- Author:
Xiao-dong TANG
1
;
Wei GUO
;
Yi GUO
;
Sha LI
;
Xinpu HOU
Author Information
- Publication Type:Journal Article
- MeSH: Alginates; administration & dosage; Animals; Capsules; Chemoembolization, Therapeutic; Chitin; administration & dosage; analogs & derivatives; Chitosan; Disease Models, Animal; Doxorubicin; administration & dosage; Glucuronic Acid; administration & dosage; Hexuronic Acids; administration & dosage; In Situ Nick-End Labeling; Neoplasms, Experimental; drug therapy; pathology; Proliferating Cell Nuclear Antigen; analysis; Rabbits
- From: Chinese Journal of Surgery 2003;41(12):940-943
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of chemoembolization with adriamycin alginate-chitosan microcapsules in the treatment of VX2 carcinoma in the extremity of the rabbit.
METHODSTwenty-four New Zealand white rabbits transplanted with VX2 carcinoma cells into the muscle tissue of the lower right thigh were divided into four groups namely groups A, B, C and D, and received regional infusion from femoral artery. Each group consisted of six rabbits: a group given natural saline (Group A), a group given adriamycin (Group B), a group given blank alginate-chitosan microcapsules (Group C) and a group given adriamycin alginate-chitosan microcapsules (Group D). Three days after treatment, all groups were examined by histology and immunohistochemical detection (TdT-mediated dUTP nick end labeling technique, TUNEL; proliferating cell nuclear antigen, PCNA).
RESULTSThe blood vessels of the tumor were almost embolized by the microcapsules. Extensive necrosis, high level of positive cells in TUNEL (60.85% +/- 5.21%) and low level in PCNA detection with in the tumors were observed in Group D.
CONCLUSIONAdriamycin alginate-chitosan microcapsules can potentially play roles in two respects, 1 to serve as embolizing agents, and 2 to serve as drug delivery vehicles for local release. Chemoembolization with microparticals is an effective treatment of malignant osseous and soft tissue sarcomas in an experimental model.
