Effects of 11,12-epoxyeicosatrienoic acid on cardioplegia and reperfusion arrhythmias in the isolated immature rabbit hearts.
- Author:
Ming-yang ZHOU
1
;
Qing-yu WU
;
Xing ZHONG
;
Cun LONG
;
Fu-xing WEN
;
Xiao-yan QIN
Author Information
- Publication Type:Journal Article
- MeSH: 8,11,14-Eicosatrienoic Acid; analogs & derivatives; pharmacology; Animals; Arrhythmias, Cardiac; prevention & control; Heart Arrest, Induced; Heart Rate; drug effects; In Vitro Techniques; Myocardial Reperfusion Injury; prevention & control; Rabbits
- From: Chinese Journal of Surgery 2003;41(10):781-784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe aim of the present study was to study the Effects of 11,12-epoxyeicosatrienoic acid (11,12-EET) on cardioplegia and reperfusion arrhythmias in the isolated perfused immature rabbit hearts.
METHODSIsolated immature rabbit hearts were randomly divided into two groups: group 1 (St. Thomas No.2 solution control n = 8) and group 2 (St. Thomas No.2 solution plus 11,12-EET n = 8). By means of Langendorff technique, these isolated rabbit hearts underwent (15 degrees C) hypothermia, 2 hours of ischemia after infusion of cardioplegic solution and 1 hour of reperfusion (37 degrees C). The mean times until the cessation of both electrical and mechanical activity were measured after infusion of cardioplegia. The same index until occurrence of both electrical and mechanical activity after reperfusion was observed too. We also measured the arrhythmias score, heart rate, coronary blood flow during the reperfusion and the myocardial water content, myocardial calcium content at the endpoint of the reperfusion period.
RESULTSThe times until electrical [(9.3 +/- 0.9) s vs (13.6 +/- 1.9) s, P < 0.01] and mechanical [(4.5 +/- 1.7) vs (7.3 +/- 2.1) s, P < 0.05] activity arrest were significantly shorter in the group 2 than those in the control group. 11,12-EET also provided significantly better myocardial water content [(84 +/- 4)% vs (90 +/- 5)%, P < 0.01], arrhythmia scores (2.03 +/- 0.83 vs 3.88 +/- 1.25, P < 0.01), coronary blood flow and myocardial calcium content [(3.22 +/- 0.33) micro mol/gram dry weight (gdw) vs (3.97 +/- 0.26) micro mol/gdw, P < 0.01] compared with control. There were no significant changes with heart rate and the mean times until occurrence of both electrical and mechanical activity after reperfusion.
CONCLUSIONSThese data suggest that 11,12-EET added to the cardioplegic solution of St. Thomas No.2 has better cardioplegia effects and lower incidence of reperfusion arrhythmias.