Somatic mutations of VHL gene and HIF-1alpha expression in primary renal clear cell carcinomas.
- Author:
Hong-feng GUO
1
;
Kan GONG
;
Shuang-mei ZOU
;
Zhi-wen ZHANG
;
Xiu-yun LIU
;
Xi NA
;
Guan WU
;
Yan-qun NA
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma, Clear Cell; genetics; pathology; Adult; Aged; Carcinoma, Renal Cell; genetics; pathology; Chromatography, Liquid; Female; Gene Expression Regulation, Neoplastic; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Immunohistochemistry; Kidney; chemistry; metabolism; pathology; Kidney Neoplasms; genetics; pathology; Male; Middle Aged; Mutation; genetics; Polymerase Chain Reaction; Transcription Factors; analysis; genetics; Tumor Suppressor Proteins; analysis; genetics; Ubiquitin-Protein Ligases; analysis; genetics; Von Hippel-Lindau Tumor Suppressor Protein
- From: Chinese Journal of Surgery 2004;42(4):196-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the significance of somatic mutations of VHL gene and hypoxia-inducible factor-1alpha (HIF-1alpha) expression in primary renal clear cell carcinoma (RCC).
METHODSMutation of VHL gene and HIF-1alpha expression were detected by means of PCR, denaturing high-performance liquid chromatography (DHPLC), direct sequencing and immunohistochemistry in 32 samples from primary renal clear cell carcinoma patients.
RESULTSIn 32 RCC samples, 17 samples (53.1%) had and 32 samples of adjacent nonmalignant renal tissue had not mutations of VHL gene expression. Twelve RCC samples (70.6%) which had mutations of VHL gene expressed HIF-1alpha, and it had significant difference to 4 RCC (26.7%) samples which didn't have mutations of VHL gene (P < 0.05).
CONCLUSIONMutations of VHL gene may play a significant role in the tumorigenesis of RCC, and HIF-1alpha expression correlates with it.
