Effect of nitric oxide donor and alpha1-receptor antagonist on proliferation/apoptosis of hyperplastic prostatic stromal cells in vitro.
- Author:
Chun-hua DENG
1
;
Hui-rong CHEN
;
Shao-peng QIU
;
Jian-zhong LIU
;
Ke-li ZHENG
;
Hua MEI
Author Information
- Publication Type:Journal Article
- MeSH: Adrenergic alpha-Antagonists; pharmacology; Apoptosis; drug effects; Dose-Response Relationship, Drug; Drug Synergism; Humans; In Situ Nick-End Labeling; Male; Nitric Oxide Donors; pharmacology; Prazosin; analogs & derivatives; pharmacology; Prostatic Hyperplasia; drug therapy; pathology; Stromal Cells; drug effects; pathology
- From: Chinese Journal of Surgery 2004;42(4):201-204
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of nitric oxide donor and alpha(1)-receptor antagonist on proliferation/apoptosis of hyperplastic prostatic stromal cells in vitro.
METHODSPrimary cultured prostatic stromal cells were treated by nitric oxide donor SNP and Terazosin, and the antiproliferative index and apoptosis index were determined by MTT assay and TUNEL respectively.
RESULTSThere was a significant dose-effect relationship between SNP and the antiproliferative effects, while Terazosin showed no antiproliferative effects and the combination of SNP and Terazosin showed no strengthen effects. Terazosin significantly induced apoptosis, but SNP showed no effect on induction of apoptosis, while there were much more effects of inducing apoptosis in the combination of Terazosin and SNP than the Terazosin alone.
CONCLUSIONSTerazosin induces apoptosis in cultured BPH stromal SMCs with little effect on the cell proliferation. SNP inhibits the proliferation of the cells without affecting apoptosis. The apoptosis induction effect is enhanced when Terazosin is combined with SNP, but they do not have an additive antiproliferative effect.
