Rapid PCR authentication Lonicera japanica.
- Author:
Chao JIANG
;
Jing-Yi HOU
;
Lu-Qi HUANG
;
Yuan YUAN
;
Min CHEN
;
Yan JIN
- Publication Type:Journal Article
- MeSH:
Alleles;
DNA Primers;
genetics;
Drug Contamination;
prevention & control;
Lonicera;
classification;
genetics;
Polymerase Chain Reaction;
methods;
Quality Control
- From:
China Journal of Chinese Materia Medica
2014;39(19):3668-3672
- CountryChina
- Language:Chinese
-
Abstract:
To simply and rapid authenticate Lonicera japanica. Rapid allele-specific PCR primer was designed base on trnL-trnF 625 G/T Single nucleotide polymorphism and the PCR reaction systems including annealing temperature was optimized; optimized results were performed to authenticate L. japanica and its 9 adulterants. When 100 x SYBR Green I was added in the PCR product of 87 degrees C initial denatured 1 min; 87 degrees C denatured 5 s, 68 degrees C annealing 5 s, 30 cycle; L. japanica visualize strong green fluorescence under 365 nm UV lamp whereas adulterants without. The results indicate rapid allele-specific PCR could authenticate L. japanica and its adulterants rapidly and simply.
