Rapid extraction of DNA from Chinese medicinal products by alkaline lysis.
- Author:
Qi ZHENG
;
Chao JIANG
;
Lu-Qi HUANG
;
Zhi-Jie ZHANG
;
Rao-Rao LI
;
Kang CHEN
;
Yuan YUAN
;
Yan JIN
- Publication Type:Journal Article
- MeSH:
Alkalies;
chemistry;
Chemical Fractionation;
methods;
DNA, Plant;
genetics;
isolation & purification;
Hydrolysis;
Plants, Medicinal;
chemistry;
classification;
genetics;
Polymerase Chain Reaction;
Sophora;
chemistry;
classification;
genetics
- From:
China Journal of Chinese Materia Medica
2014;39(19):3678-3683
- CountryChina
- Language:Chinese
-
Abstract:
The study is aimed to explore a rapid method to extract DNA from fried Chinese medicinal products. The alkaline lysis buffer was made of sodium hydroxide, 1% PVP and 1% TritonX-100 and Tris-HCl solution was neutralized, through heat cracking and neutralization two step to extract DNA from processed and prepared products of traditional Chinese medicine. Then universal primes were used to amplify PCR products for fired Chinese medicinal materials. The results indicated the optimized alkaline lysis method for extracting DNA is quick and easy. Extracting of the different processed Sophora japonica of DNA concentration was (420.61 ± 123.91) g x L(-1). Using 5% Chelex-100 resin purification can improve the DNA concentration. Our results showed that the optimized alkaline lysis method is suitable for Chinese medicinal materials for quickly DNA extraction.
