Study on fluorescence sequencing typing technology identification of raw materials in liuwei dihuang pill.
- Author:
Zhan-Hu CUI
;
Lu-Qi HUANG
;
Yuan YUAN
;
Min-Hui LI
;
Chao JIANG
;
Li-She ZHOU
- Publication Type:Journal Article
- MeSH:
DNA Primers;
chemistry;
genetics;
DNA, Plant;
chemistry;
genetics;
Drugs, Chinese Herbal;
chemistry;
standards;
Fluorescent Dyes;
chemistry;
Plants, Medicinal;
chemistry;
genetics;
Polymerase Chain Reaction;
instrumentation;
methods;
Quality Control;
Staining and Labeling
- From:
China Journal of Chinese Materia Medica
2014;39(19):3695-3700
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, Liuwei Dihuang pill was used to study the identification of Chinese patent medicine by fluorescence sequencing typing technology. The DNA of Paeonia suffruticosa was used as template to amplify by five pair of FAM fluorescence labeling primers. Then, the amplified products were sequenced. The sequencing results were analyzed by GeneMarker V1.80 to screen the best fluorescence labeling primers. As a result, psbA-trnH fluorescence labeling primer was used to identify the raw materials of Liuwei Dihuang pill. The results showed that three kinds of raw plant medicinal materials in Liuwei Dihuang pill were able to be correctly identified by psbA-trnH fluorescence labeling primer. The fluorescence sequencing typing technology can stably and accurately distinguish raw medicinal materials in Chinese patent medicine.
