Anti-arthritic and anti-oxidative effect of ethyl acetate fraction of Blumea balsamlfera residues in rat adjuvant-induced arthritis.
- Author:
Yan XIA
;
Jian ZUO
;
Xiang LI
;
Jian-Wei CHEN
- Publication Type:Journal Article
- MeSH: Acetates; Animals; Anti-Inflammatory Agents, Non-Steroidal; administration & dosage; isolation & purification; Arthritis, Experimental; blood; drug therapy; metabolism; Asteraceae; chemistry; Drugs, Chinese Herbal; administration & dosage; isolation & purification; Humans; Interleukin-1; blood; Interleukin-6; blood; Male; Malondialdehyde; blood; Oxidative Stress; drug effects; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha; blood
- From: China Journal of Chinese Materia Medica 2014;39(19):3819-3823
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of ethyl acetate fraction of Blumea balsamifera (BBE) residue on treating rats of adjuvant arthritis (AA) and its mechanism.
METHODThe rats were immunized with the Freund's complete adjuvant (FCA). After modeling, 28 days' treatment with BBE was performed. During the experimental process, rat mass, toe girth, arthritic index (AI), proliferation of immune organs and pathological section were measured. After treatment, blood samples were collected through fossa orbitalis vein for detection of serum SOD, MDA, GSH, NO, OH*, ALP, AST, ALT, NAG and SA content using colorimetric method and IL-1, IL-6, TNF-α content using ELISA method.
RESULTAdministration with BBE (high dose) could significantly ameliorate joint swelling and arthritis index, effectively inhibit synovial hyperplasia, down-regulate the levels of MDA, NO, OH*, ALP, AST, ALT, NAG, SA, IL-1, IL-6, TNF-α and up-regulate the SOD and GSH levels in serum.
CONCLUSIONThe results suggested BBE possesses substantial anti-arthritis and antioxidant activities.