Construction of Saccharomyces cerevisiae cell factories for lycopene production.
- Author:
Ming-Yu SHI
;
Liu YI
;
Dong WANG
;
Fu-Ping LU
;
Lu-Qi HUANG
;
Zhu-Bo DAI
;
Xue-Li ZHANG
- Publication Type:Journal Article
- MeSH:
Bacterial Proteins;
genetics;
metabolism;
Biosynthetic Pathways;
Carotenoids;
biosynthesis;
Genes, Synthetic;
Genetic Engineering;
Pantoea;
enzymology;
genetics;
Saccharomyces cerevisiae;
genetics;
metabolism
- From:
China Journal of Chinese Materia Medica
2014;39(20):3978-3985
- CountryChina
- Language:Chinese
-
Abstract:
For microbial production of lycopene, the lycopene synthetic genes from Pantoea agglomerans were integrated into Saccharomyces cerevisiae strain BY4742, to obtain strain ZD-L-000 for production of 0.17 mg · L(-1) lycopene. Improving supplies of isoprenoid precursors was then investigated for increasing lycopene production. Four key genes were chosen to be overexpressed, inclu- ding truncated 3-hydroxy-3-methylglutaryl-CoA reductase gene (tHMG1), which is the major rate-limiting enzyme in the mevalonate (MVA) pathway, a mutated global regulatory factor gene (upc2.1), a fusion gene of FPP synthase (ERG20) and endogenous GGPP synthase (BTS1), which is a key enzyme in the diterpenoid synthetic pathway, and GGPP synthase gene (SaGGPS) from Sulfolobus acidocaldarius. Over-expression of upc2.1 could not improve lycopene production, while over-expression of tHMGI , BTS1-ERG20 and SaGGPS genes led to 2-, 16. 9- and20. 5-fold increase of lycopene production, respectively. In addition, three effective genes, tHMG1, BTS1-ERG20 and SaGGPS, were integrated into rDNA sites of ZD-L-000, resulting in strain ZD-L-201 for production of 13.23 mg · L(-1) lycopene, which was 77-fold higher than that of the parent strain. Finally, two-phase extractive fermentation was performed. The titer of lycopene increased 10-fold to 135.21 mg · L(-1). The engineered yeast strains obtained in this work provided the basis for fermentative production of lycopene.
