Study on quality standard for Humulus scandens.
- Author:
Fan XIE
;
Ying-Chun WU
;
Yi-Ming LI
;
Hong XU
;
Rui WANG
;
Zheng-Tao WANG
- Publication Type:Journal Article
- MeSH:
China;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
standards;
Humulus;
anatomy & histology;
chemistry;
Quality Control
- From:
China Journal of Chinese Materia Medica
2014;39(20):3986-3990
- CountryChina
- Language:Chinese
-
Abstract:
To control the quality of Humulus scandens, the quality standard was established in this study. According to the method recorded in the Appendix of Chinese Pharmacopoeia (2010 Edition) , the water and ash inspections were carried out. The component luteoloside and cosmosiin in Humulus scandens were identified and assayed by TLC and HPLC. The results showed a strong characteristics microscopic of Humulus scandens, and trichoromethane-methanol-formic acid (10: 3: 0. 3) as the mobile phase of TLC, the spots at 365 nm with a UV lamp was clear. The 16 batches of samples were analyzed by HPLC with a gradient elution of acetonitrile and phosphate solution (0.2%) at a flow rate of 1.0 mL · min(-1) and detected at 350 nm. The content of luteoloside was 0.015%- 0.651% (average 0.148%); the content of cosmosiin was 0.003%-0.118% (average 0.036%). The linear calibration curve of luteoloside and cosmosiin was acquired in the ranges of 0.011-0.364 g · L(-1) (r = 1.000 0) and 0.003-0.096 g · L(-1) (r = 1.000 0), respectively. The average recovery was 100.5% and 98.5%, respectively. The methods are convenient and reliable, which can be ap- plied for quality assessment of Humulus scandens.
