Fungal composition in massa medicata fermentata based on culture dependent method and independent PCR-SSCP technique.
- Author:
Juan CHEN
;
Xiao-lin JIAO
;
Chun-yong YANG
;
Mei-fang SONG
;
Wei-wei GAO
- Publication Type:Journal Article
- MeSH: Fermentation; Fungi; isolation & purification; Medicine, Chinese Traditional; Polymerase Chain Reaction; methods; Polymorphism, Single-Stranded Conformational; Tubulin; genetics
- From: China Journal of Chinese Materia Medica 2014;39(21):4169-4173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the fungal composition in Massa Medicata Fermentata based on culture dependent method and independent PCR-SSCP technique.
METHODFungi were directly isolated from Massa Medicata Fermentata samples. The obtained strains were identified according to morphology and DNA sequence. Meanwhile the total fungal DNA was extracted from Massa Medicata Fermentata samples, the cultural independent PCR-SSCP technique based on β-tubulin gene were used to identify the mycobiota.
RESULTAccording to cultural method, Aspergillus flavus and Rhizopus oryzae were present in Massa Medicata Fermentata samples, while A. flavus and A. niger were present in fried Massa Medicata Fermentata samples. In contrast, 5 species were obtained by PCR-SSCP technique, A. flavus was overlapped with fungal taxa derived from culture dependent method; A. ambiguu and A. s ivoriensis were dominant with relative abundance of 57% and 35% respectively, while the relative abundance of A. flavus was as low as 4%. None species was obtained from fried Massa Medicata Fermentata samples.
CONCLUSIONPCR-SSCP based on β-tubulin gene could distinguish fungi into species, culture dependent method combined with culture independent method could better understand the fungal composition associated with Massa Medicata Fermentata fermentation.