Inhibition mechanism of Qingluo Tongbi Granule () on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats.
- Author:
Tian-yang LIU
1
;
Ling-ling ZHOU
;
Cong ZHOU
;
Zhang-pu LIU
;
Chen CHEN
;
Zhe FENG
;
Xue-ping ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; adverse effects; Animals; Arthritis, Experimental; pathology; Cell Differentiation; drug effects; Cells, Cultured; Coculture Techniques; Down-Regulation; drug effects; Drugs, Chinese Herbal; pharmacology; Fibroblasts; pathology; Male; Monocytes; pathology; Osteoclasts; cytology; drug effects; physiology; Rats; Rats, Sprague-Dawley; Synovial Membrane; pathology
- From: Chinese journal of integrative medicine 2015;21(4):291-298
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the mechanism underlying the inhibitory effect of Qingluo Tongbi Granule (, QTG) on osteoclast differentiation in rheumatoid arthritis in rats.
METHODSFibroblast and monocyte co-culture were used to induce osteoclast differentiation in adjuvant-induced arthritic (AIA) rats. Serum containing QTG was prepared and added to the osteoclasts, and activation of the tumor necrosis factor receptor-associated factor 6/mitogen-activated protein kinase/nuclear factor of activated T cells, cytoplasmic1 (TRAF6/MAPK/NFATc1) pathways was examined.
RESULTSThe induced osteoclasts were multinucleated and stained positive for tartrate-resistant acid phosphatase (TRAP) staining. Serum containing QTG at 14.4, 7.2 or 3.6 g/kg inhibited the activation of TRAF6, extracellular regulated protein kinase (ERK)1/2, c-Jun N-terminal kinase (JNK) and p38 and decreased the percentage of cells with nuclear NFATc1 in a dose-dependent manner, the high and middle doses exhibited clear inhibitory activity (P<0.01 and P<0.05, respectively). After the addition of MAPK inhibitors, the NFATc1 expression showed no significant difference compared with the control group (P>0.05).
CONCLUSIONSSerum containing QTG could generally inhibit the TRAF6/MAPK pathways and possibly inhibit the NFATc1 pathway. In addition, QTG may regulate other signaling pathways that are related to osteoclast differentiation and maturation.
