Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells.
- Author:
Li-hua YIN
1
;
Wen-xiao CHENG
1
;
Zi-shun QIN
1
;
Ke-mo SUN
1
;
Mei ZHONG
1
;
Jia-kui WANG
1
;
Wei-yue GAO
1
;
Zhan-hai YU
2
Author Information
- Publication Type:Journal Article
- Keywords: Chinese medicine; ginsenoside Rg-1; human periodontal ligament stem cell; osteogenic differentiation; proliferation
- MeSH: Adolescent; Alkaline Phosphatase; metabolism; Biomarkers; metabolism; Calcification, Physiologic; drug effects; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cell Separation; Cell Shape; drug effects; Cells, Cultured; Flow Cytometry; Ginsenosides; pharmacology; Humans; Osteoblasts; drug effects; metabolism; Osteogenesis; drug effects; genetics; Periodontal Ligament; cytology; Real-Time Polymerase Chain Reaction; Stem Cells; cytology; drug effects; enzymology; Time Factors; Young Adult
- From: Chinese journal of integrative medicine 2015;21(9):676-681
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to explore the possible application on the alveolar bone regeneration.
METHODSTo determine the optimum concentration, the effects of ginsenoside Rg-1 ranging from 10 to 100 μmol/L were evaluated by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide, alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2, collagen alpha-2(I) chain, osteopontin, osteocalcin protein were examined using real-time polymerase chain reaction.
RESULTSCompared with the control group, a certain concentration (10 μmol/L) of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs (P<0.05). However, concentrations that exceeds 100 μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control.
CONCLUSIONGinsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol/L.
