- Author:
Tan WANG
1
;
Xiang-Gen ZHONG
;
Yu-Hang LI
;
Xu JIA
;
Shu-Jing ZHANG
;
Yu-Shan GAO
;
Miao LIU
;
Ruo-Han WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchoalveolar Lavage Fluid; cytology; Chemokines; metabolism; Disease Models, Animal; Emodin; chemistry; pharmacology; therapeutic use; Female; Gene Expression Regulation; drug effects; Heme Oxygenase-1; genetics; metabolism; Immunoglobulin E; blood; Interleukins; genetics; metabolism; Leukocytes; drug effects; metabolism; Lung; drug effects; metabolism; pathology; Matrix Metalloproteinase 9; genetics; metabolism; Mice, Inbred C57BL; Ovalbumin; Pneumonia; blood; drug therapy; pathology; Protective Agents; pharmacology; therapeutic use; RNA, Messenger; genetics; metabolism
- From: Chinese journal of integrative medicine 2015;21(6):431-437
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate whether emodin exerts protective effects on mouse with allergic asthma.
METHODSA mouse model of allergic airway inflflammation was employed. The C57BL/6 mice sensitized and challenged with ovalbumin (OVA) were intraperitoneally administered 10 or 20 mg/kg emodin for 3 days during OVA challenge. Animals were sacrificed 48 h after the last challenge. Inflammatory cell count in the bronchoalveolar lavage fluid (BALF) was measured. The levels of interleukin (IL)-4, IL-5, IL-13 and eotaxin in BALF and level of immunoglobulin E (IgE) in serum were measured with enzyme-linked immuno sorbent assay kits. The mRNA expressions of IL-4, IL-5, heme oxygenase (HO)-1 and matrix metalloproteinase-9 (MMP-9) were determined by real-time quantitative polymerase chain reaction.
RESULTSEmodin induced significant suppression of the number of OVA-induced total inflammatory cells in BALF. Treatment with emodin led to significant decreases in the levels of IL-4, IL-5, IL-13 and eotaxin in BALF and total IgE level in serum. Histological examination of lung tissue revealed marked attenuation of allergen-induced lung eosinophilic inflammation. Additionally, emodin suppressed IL-4, IL-5 and MMP-9 mRNA expressions and induced HO-1 mRNA expression.
CONCLUSIONEmodin exhibits anti-inflammatory activity in the airway inflammation mouse model, supporting its therapeutic potential for the treatment of allergic bronchial asthma.