Effect of Salvia miltiorrhiza monomer IH764-3 on MMP-13 and TIMP-1 by downregulating the expression of focal adhesion kinase in hepatic stellate cell stimulated by H2O2.
- Author:
Li LIU
1
;
Hui-qing JIANG
;
Xiao-lan ZHANG
;
Dong-qiang ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Down-Regulation; Drugs, Chinese Herbal; pharmacology; Focal Adhesion Protein-Tyrosine Kinases; metabolism; Hepatic Stellate Cells; drug effects; metabolism; Hydrogen Peroxide; adverse effects; Matrix Metalloproteinase 13; metabolism; Rats; Salvia miltiorrhiza; chemistry; Tissue Inhibitor of Metalloproteinase-1; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(4):482-486
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of IH764-3 on the expression of MMP-13 and TIMP-1 by H2O2-stimulated hepatic stellate cell and the alteration of FAK during this process.
METHODSThe expression of MMP-13 and FAK mRNA was examined by RT-PCR. TIMP-1 mRNA was analyzed by in-situ hybridization. FAK and TIMP-1 were evaluated at protein level through Western blotting method.
RESULTSBeing incubated for 2 h, compared with control group, MMP-13 mRNA was upregulated by IH764-3, but TIMP-1 transcription was reduced in a dose-dependent manner, accompanied with the decrease of FAK mRNA. The expression of TIMP-1 and FAK protein in HSC also decreased after being exposed by IH764-3 for 24 h.
CONCLUSIONIH764-3 can induce the expression of MMP-13 and inhibit the expression of TIMP-1. Down-regulating the expression of FAK mRNA may be one of its mechanisms.
