Effect of glucose-6-phosphate dehydrogenase on intracellular gsh level in Raji cells during oxidative stress.
- Author:
De-tai ZHANG
1
;
Li-hua HU
;
Yu-zhen YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Glucosephosphate Dehydrogenase; metabolism; Glutathione; metabolism; Glutathione Peroxidase; metabolism; Humans; Oxidation-Reduction; Oxidative Stress; Receptors, Peptide; metabolism
- From: Chinese Journal of Applied Physiology 2007;23(4):487-490
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore a role of G6PD in replenishment of intracellular GSH during oxidative stress.
METHODSIn vitro Raji cell was cultured, intracellular GSH levels and G6PD, GR, GPX activities were determined at different time points after PMS treatment when G6PD activity was inhibited or not by DHEA.
RESULTSIntracellular GR, GPX, G6PD activities elevated significantly combined with GSH level decreased dramatically before 30 minutes, replenished gradually after 30 minutes and restore normal levels about 6 h after PMS treatment when G6PD was not inhibited. No change in GR and significant increase in GPX activity were shown following depleted GSH after PMS treatment when G6PD was inhibited by DHEA.
CONCLUSIONG6PD contributes to replenish intracellular GSH and is a critical factor regulating GSH levels during oxidative stress.
