Comparison between anti-ouabain egg yolk(IgY) and rabbit antibody(IgG) in enzyme-linked immunosorbent assay.
- Author:
Ming-juan ZHANG
1
;
Jun YANG
;
Heng GE
;
Lei QIANG
;
Zong-ming DUAN
;
Cong-xia WANG
;
Rong WANG
;
Zhuo-rern LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibody Specificity; Chickens; immunology; Cross Reactions; Egg Yolk; immunology; Enzyme-Linked Immunosorbent Assay; methods; Immunoglobulin G; immunology; Immunoglobulins; immunology; Male; Ouabain; analysis; Rabbits
- From: Chinese Journal of Applied Physiology 2007;23(4):505-508
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo improve specificity and accuracy of endogenous ouabain measurement assay.
METHODSAnti-ouabain polyclonal antibody egg yolk (IgY) and anti-ouabain rabbit antibody (IgG) were prepared respectively. In the presence of two kinds of antibody, then the specificity and accuracy of enzyme-linked immunosorbent assay (ELISA) were compared.
RESULTSThe ELISA, in the presence of IgY, provided a sensitivity of the average intraassay coefficient of variation(CV) was 2.03%, and the inter-assay CV was 2.34% respectively. In contrast, IgG were 2.83% and 3.29%. No significant interferences were observed with hydrocortisone and dexamethasone. There was 3.45% vs. 5.95%, 3.20% vs. 5.20% of crossreaction with cedilanid and digoxin.
CONCLUSIONThe specificity and accuracy of ELISA, in which IgY was used, were more better than IgG.
