Effect of musk soluble components on the growth, the differentiation and the transfection efficiency of rat neural stem cells (NSC) in vitro.
- Author:
Qi SHEN
1
;
Zhi-Hua GUO
;
De-Xiang LI
;
Tie-Qiao WEN
;
Fu-Xue CHEN
;
Hong-Sheng SONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; cytology; Cell Differentiation; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Culture Media; Fatty Acids, Monounsaturated; chemistry; Female; Fetus; Male; Materia Medica; pharmacology; Neural Stem Cells; cytology; Rats; Rats, Sprague-Dawley; Transfection
- From: Chinese Journal of Applied Physiology 2008;24(1):25-28
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of musk soluble components on the growth, the differentiation and the transfection efficiency of rat neural stem cell (NSC) in vitro.
METHODSThe growth and the differentiation of rat NSC were observed when musk soluble components were added into the culture medium of NSC. Meanwhile, the pEGFP-C1, which expressed the enhanced GFP protein, was transfected into the NSC by method of electro- transfection.
RESULTSWhen NSC was treated with musk soluble components, the neurites were outgrowth around NSC and attached to the plate, and the neural spheres were disassociated. The glia-like cells appeared at the concentration of 0.3 per thousand. When the concentration of musk soluble components was lower than 3 per thousand, the transformative cells could recover. Furthermore, the efficiency of transfection pEGFP into NSC was remarkably increased after the treatment with musk.
CONCLUSIONAfter the treatment of NSC with musk soluble components, the neural spheres were disassociated, and then attached to the plate. Musk soluble components could induce NSC differentiation into glia-like cells and improve the transfection efficiency of pEGFP-C1 in vitro.
