The expression and control of telomerase in preantral ovary.
- Author:
Jing ZHANG
1
;
Yue-Hui ZHENG
;
Li-Ping ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Chorionic Gonadotropin; pharmacology; Estradiol; physiology; Female; Follicle Stimulating Hormone; pharmacology; Granulosa Cells; cytology; enzymology; Ovary; cytology; enzymology; Rats; Rats, Sprague-Dawley; Telomerase; metabolism; physiology; Verapamil; pharmacology
- From: Chinese Journal of Applied Physiology 2008;24(1):86-89
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the expression and control of telomerase in rat preantral ovary.
METHODSAdded different factors to the preantral ovarian granulosa cells, then using TRAP-ELISA to analyze the expression of telomerase.
RESULTSTelomerase activation was detected in granulosa cells. Telomerase activation could be significantly enhanced by hcG, FSH, verapamil and dbcAMP, and it was significantly reduced by antisense-c-myb ODN treatment. We also used radioimmunoassay to determine proterone and estrogen in these cell culture mediums. It was found that these two hormones' secretion were raised under verapamil and FSH; no change under hcG and dbcAMllted with telomerase activity. In MTT assay, the antisense hTERT ODN could significantly inhibited the proliferation of ovarian granulosa cells.
CONCLUSIONIt is suggested that telomerase activation is present in ovary antral granulosa cell. Anti-c-myb might inhibit telomerase activation, while FSH, hcG, verapamil, dbcAMP might enhance the telomerase activation.
