AIMTo observe the preventive function of cortex mori for peripheral nervous lesion at the early stage in diabetes rats, and probe into the mechanism of that formula.

METHODSSet up the diabetes rat model evoked by alloxan. According to different blood sugar values, randomly divide the rats into normal group, model group, cortex mori(high dosage and low dosage) group and methylcobalamin control group, respectively administer the rats with saline and cortex mori of different dosages by ig (1.875 g/kg, 0.625 g/kg), while 300 microg/kg methylcobalamin for control group, one time each day. Two months after the administration, determine the FBG, body weight, SOD and MDA in blood serum of rats in each group, observe the changes on final product of glycosylation, CGMP and CAMP of sciatic nerve and the synapsin of rats' sciatic nerves. Conduct the pathological observation on area of myelin sheath, extramedullary fiber and the cross section of myelin sheath of sciatic nerves. And observe the changes of ultrafine form of sciatic nerve through transmission electron microscope. In the mean time, determine the MNCV, SNVC and SL, and the tail-flicking test should be undertaken for checking the sensory nerve.

RESULTSCortex mori can effectively enlarge the area of myelin sheath, extramedullary fiber and the cross section of myelin sheath.

CONCLUSIONCortex mori can obviously ease up the pathological changes of peripheral nerve at the early stage of the diabetes rats, and the overall curative effect is better than that of methylcobalamin.