Interleukin-8 inhibits clonal expansion of 3T3-L1 preadipocyte during differentiation.
- Author:
Hua ZHOU
1
;
Xi YANG
;
Ya-Ou ZHANG
;
Guo-Ping CAI
Author Information
- Publication Type:Journal Article
- MeSH: 3T3-L1 Cells; Adipocytes; cytology; metabolism; Animals; Cell Cycle; Cell Differentiation; Cell Proliferation; drug effects; Interleukin-8; pharmacology; Mice
- From: Chinese Journal of Applied Physiology 2008;24(2):243-247
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of interleukin-8 (IL-8) on the differentiation and clonal expansion of 3T3-L1 preadipocyte during the differentiation period.
METHODSThe morphological changes of 3T3-L1 cells during differentiation after the treatment of IL-8 was observed by Oil-Red O staining. Glycerol-3-phosphate dehydrogenase (GPDH) activity was measured by a spectrophotometric method. MTT method and 3H-TdR incorporation were applied to examine the changes of cell proliferation and DNA synthesis in clonal expansion of 3T3-L1 cells. Cell cycle analysis was taken by flow cytometry.
RESULTSIL-8 could inhibit the differentiation and GDPH activity in a dose dependent manner. IL-8 decreased the cell proliferation and DNA synthesis in clonal expansion after induction. Also, the proportion of cells in G1 phase was increased and that of cells in S and G2 phase was declined after the treatment of IL-8.
CONCLUSIONIL-8 inhibits the differentiation of 3T3-L1 preadipocytes by decreasing the clonal expansion of the cells.
