Research progress in multi-enzyme regulation of genetically engineered bacteria producing lycopene.

Jiali XU; Siyi Zuo; Chengjia Xie; Ling Jiang; Shuang LI; He Huang; Xian XU

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Research progress in multi-enzyme regulation of genetically engineered bacteria producing lycopene.

Author: Jiali XU ¹ ; Siyi ZUO ¹ ; Chengjia XIE ² ; Ling JIANG ³ ; Shuang LI ¹ ; He HUANG ⁴ ; Xian XU ⁴
Author Information

1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China.
2. College of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou 225127, Jiangsu, China.
3. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, Jiangsu, China.
4. College of Pharmacy, Nanjing Tech University, Nanjing 211816, Jiangsu, China.
Publication Type:Journal Article
Keywords: genetic engineering; lycopene; multi-enzyme regulation; multi-fragment assembly; scaffold
From: Chinese Journal of Biotechnology 2017;33(4):552-564
CountryChina
Language:Chinese
Abstract: Lycopene plays a crucial role in the biosynthesis pathway of 2-methyl-derythritol-4-phosphate (MEP) and mevalonic acid (MVA). It is a representative product of isoprenoid family, and a typical product of multi-enzyme catalytic reaction in organism. In this paper, we first introduced the general regulation methods in multi-enzyme synthesis reaction, including the construction of multi gene co-expression plasmid, gene order regulation, promoter and ribosome binding site regulation, gene knockout and replacement, aiming at the optimization strategies of multi-enzyme catalytic reaction in lycopene synthesis pathway. Meanwhile, we introduced several new regulation methods in multi-enzyme reaction, including multi-fragment assembly technology, artificial scaffold self-assembly methods and so on. At last, we summarized the application of these multi-enzyme regulation methods in lycopene synthesis. These methods provide a great inspiration and research foundation for the construction of lycopene-producing strains with high yield.