Advance of heterologous expression study of eukaryote-origin laccases.

Author: Na NING ¹ ; Huijun TAN ¹ ; Xinxin SUN ¹ ; Jinfeng NI ¹
Author Information

1. State Key Laboratory of Microbial Technology, Shandong University, Ji'nan 250100, Shandong, China.
Publication Type:Journal Article
Keywords: enzyme activity; eukaryote-origin laccases; heterologous expression; stability
From: Chinese Journal of Biotechnology 2017;33(4):565-577
CountryChina
Language:Chinese
Abstract: Laccases are enzymes belonging to the group of multi-copper oxidases. These enzymes are widely distributed in insects, plants, fungi and bacteria. In general, laccases can oxidize an exceptionally high number of substrates, so they have broad applications in textile, pulp, food and the degradation of lignin. However, low yield, low activity and thermo-instability of laccase in nature limit the application of laccase. High efficient heterologous expression of the protein is an effective way for solving this problem. Here, we summarize the research advances of heterologous expression of eukaryote-origin laccases. We focus on the overexpression of eukaryote-origin laccases using different expression system and the method for improving the production yield and enzyme activity in yeast cells. Information provided in this review would be helpful for researchers in the field.