Inhibition of Fusarium graminearum by silver nanoparticles.

Qinqin LI; Yinghu Zhao; Li Gao; Qianqian Hou; Fang Wang; Wanli Jia; Yingyong Wang

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Inhibition of Fusarium graminearum by silver nanoparticles.

Author: Qinqin LI ¹ ; Yinghu ZHAO ¹ ; Li GAO ¹ ; Qianqian HOU ¹ ; Fang WANG ¹ ; Wanli JIA ¹ ; Yingyong WANG ²
Author Information

1. School of Chemical and Environmental Engineering, North University of China, Taiyuan 030051, Shanxi, China.
2. State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, Shanxi, China.
Publication Type:Journal Article
Keywords: Fusarium graminearum; antifungal activity; inhibiting mechanism; silver nanoparticles
From: Chinese Journal of Biotechnology 2017;33(4):620-629
CountryChina
Language:Chinese
Abstract: Silver nanoparticles were prepared by chemical reduction. Fusarium graminearum was used as the test strain. To study the inhibition of F. graminearum by silver nanoparticles, we studied the activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and the contents of osmotic adjustment substances soluble protein, soluble sugar and malonaldehyde (MDA) in F. graminearum. Silver nanoparticles inhibited F. graminearum and the inhibitory effect was increased with the concentration of silver nanoparticles. The inhibition rate of 10 μg/mL silver nanoparticles was more than 90% and EC50 was 0.59 μg/mL. When the treating time prolonged (2, 4, 6, 8 and 10 h), the activity of SOD, CAT and POD increased firstly and then declined. SOD, POD and CAT reached the maximum at 4 hours, and decreased to minimum at 10 hours. Silver nanoparticles also increased the MDA content and reduced the soluble sugar and protein contents in pathogens. These results indicated that cell integrity was destroyed in the presence of silver. This may be one of the inhibiting mechanisms of silver nanoparticles on the growth of F. graminearum.