Production of gastric-mucosa protective collagen Ⅲ by Pichia pastoris.

Author: Weina LI ¹ ; Zifang SHANG ¹ ; Zhiguang DUAN ¹ ; Linbo LI ¹ ; Jing HE ¹ ; Daidi FAN ¹
Author Information

1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China.
Publication Type:Journal Article
Keywords: Pichia pastoris; gastric tissue; human collagen (Ⅲ); response surface methodology
From: Chinese Journal of Biotechnology 2017;33(4):672-682
CountryChina
Language:Chinese
Abstract: To improve collagen production by recombinant Pichia pastoris, we applied Placket-Burman and Box-Behnken design to optimize the fermentation medium. Through Placket-Burman design, three variables in the medium (concentration of yeast extract, peptone and glycerol) were selected for having significant effect on cell dry weight. Through Box-Behnken design regression coefficients analysis, a secondary degree polynomial equation was established, and the optimum levels of the three variables were yeast extract 1.13%, peptone 1.61% and glycerol 0.86%. During the growth period, an average cell dry weight of 4.41 g/L was obtained after 12 h fermentation, increased by 26%. Through high density fermentation, the production of recombinant human collagen (Ⅲ) was up to 4.71 g/L in 22 L fermentor. The recombinant human collagen (Ⅲ) exhibited good results to repair acetic acid induced gastric ulcer in rats.