Challenges in large-scale synthetic gene circuits design.

Author: Lei WEI ¹ ; Ye YUAN ¹ ; Xiaowo WANG ¹
Author Information

1. Department of Automation, Ministry of Education Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China.
Publication Type:Journal Article
Keywords: competition effect; gene expression noise; rational design; synthetic gene circuit
From: Chinese Journal of Biotechnology 2017;33(3):372-385
CountryChina
Language:Chinese
Abstract: As the scale of synthetic gene circuits grows with sophisticated functions, rational design appears to be a bottleneck to develop synthetic biological systems. In this review, we summarized the impact of gene expression noise and competition effect on the performance of synthetic gene circuits. We also summarized recent progresses on rational design approaches, such as digital-analog circuits, network topologies design, and information-theory-based optimization approaches. Finally, we discussed future directions for rational design of synthetic gene circuits.