Progress in digital PCR technology and application.
- Author:
Jiaqi LIN
1
;
Guocheng SU
1
;
Wenjin SU
1
;
Changyi ZHOU
1
Author Information
- Publication Type:Journal Article
- Keywords: absolute quantification; copy number variation detection; digital PCR; genetically modified food detection; microbial detection; mutation detection; poisson distribution
- From: Chinese Journal of Biotechnology 2017;33(2):170-177
- CountryChina
- Language:Chinese
- Abstract: Digital PCR is an emerging analysis technology for absolute quantification after realtime-PCR. Through digital PCR, single DNA molecules are distributed into isolated reactions, and the product with fluorescence signal can be detected and analyzed after amplification. With the advantages of higher sensitivity and accuracy, digital PCR, independent of a standard curve, is developing rapidly and applied widely to the next generation sequencing and detection fields, such as gene mutation, copy number variation, microorganism, and genetically modified food. In this article, we reviewed the quantitative method and research progress of digital PCR technology in the main application fields.
