Metabolic engineering of Saccharomyces cerevisiae for production of glucaric acid.

Author: Xu GONG ¹ ; Ye LIU ¹ ; Cui WANG ¹ ; Jianghua LI ¹ ; Zhen KANG ¹
Author Information

1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China.
Publication Type:Journal Article
Keywords: Saccharomyces cerevisiae; glucaric acid; metabolic engineering; myo-inositol-1-phosphate synthase; phosphofructokinase
From: Chinese Journal of Biotechnology 2017;33(2):228-236
CountryChina
Language:Chinese
Abstract: Glucaric acid, a high value-added organic acid, is widely used in food, pharmaceutical and chemical industries. For microbial production of glucaric acid in Saccharomyces cerevisiae, we constructed a synthetic glucaric acid biosynthetic pathway by coexpressing the genes encoding myo-inositol oxygenase from mice and uronate dehydrogenase from Pseudomonas putida. Moreover, myo-inositol-1-phosphate synthase was identified as a rate-limiting enzyme in glucaric acid pathway and was upregulated, resulting in the production of glucaric acid of (107.51±10.87) mg/L, a 2.8-fold increase compared to the parent strain. Then, by repressing the activity of phosphofructokinase, the concentration of glucaric acid further increased to (230.22±10.75) mg/L. The strategy could be further used to construct cell factories for glucaric acid production.