Effect of ctpxa1 gene deletion in Candida tropicalis on long chain dicarboxylic acid accumulation.

Author: Cheng CHENG ¹ ; Junqing WANG ¹ ; Tengfei WANG ¹ ; Xiaohui YANG ¹ ; Ruiming WANG ¹
Author Information

1. College of Biological Engineering, Qilu University of Technology, Jinan 250353, Shandong, China.
Publication Type:Journal Article
Keywords: Candida tropicalis; ctpxa1p gene; long chain dicarboxylic acid; peroxisomes
From: Chinese Journal of Biotechnology 2017;33(2):237-246
CountryChina
Language:Chinese
Abstract: Candida tropicalis uses alkanes and fatty acids to produce long chain dicarboxylic acids. However, the yield can be affected by β-oxidation in peroxisomes. Pxa1p was a membrane protein of Saccharomyces cerevisiae peroxisomes. Pxa1p and Pxa2p form a dimer that is involved in transporting of long chain fatty acids into peroxisomes, but the similar transporting system of Candida tropicalis has not yet been reported. In this study, a ctpxa1 gene deletion strain named C. tropicalis 1798-pxa1 was constructed by homologous single exchange method using PCR fragment. The expression of ctpxa1 gene in C. tropicalis 1798, C. tropicalis 1798-pxa1 was detected by semi-quantitative RT-PCR, and the ratio of gray value was 2.03, implying that the expression of ctpxa1 in C. tropicalis 1798-pxa1 was weakened. After 144 h fermentation, the dodecanedioic acid production of C. tropicalis 1798-pxa1 was increased 94.3% than the former strain, the maximum yield was 10.3 g/L.