Design and fabrication of a microfluidic chip for the co-culture of three cell types.

Author: Shuaichao WANG ¹ ; Yuqing GE ² ; Lei WU ² ; Hui GUO ² ; Shiping YANG ¹ ; Qinghui JIN ¹
Author Information

1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
2. State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Publication Type:Journal Article
Keywords: co-culture of cell; micro dam and micro gap; microfluidic chip
From: Chinese Journal of Biotechnology 2017;33(2):294-300
CountryChina
Language:Chinese
Abstract: Here a microfluidic chip with 'micro-dam' and 'micro-gap' has been designed and fabricated. It could isolate different cells and flow of medium in each region. It was found that the chip could realize the cells co-culture and patterning of human lung adenocarcinoma cell (A549), human embryonic lung fibroblast (HLF-1) and human endothelial cells (HUVECs), respectively. After 72 hours of culture, three kinds of cells grew well. It provided a developing technical platform for cell related research.