Application of inteins in building protein affinity purification system.

Author: Shujing WANG ¹ ; Bingyou CHEN ¹ ; Yujun WANG ¹ ; Lili FENG ¹ ; Haifeng XIA ¹
Author Information

1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Publication Type:Journal Article
Keywords: affinity purification system; cleavage reaction; continuous inteins; split inteins
MeSH: Chromatography, Affinity; Inteins; Protein Splicing; Proteins; isolation & purification
From: Chinese Journal of Biotechnology 2016;32(9):1175-1184
CountryChina
Language:Chinese
Abstract: Intein is a part of polypeptide in the premature protein with the capability of self-splicing, which is widely applied in protein purification, protein conjuction, cyclopeptide preparation, protein labeling and biosensor. In this review, we summarized the development of intein used in protein purification, discussed intein-mediated chromatographic and non-chromatographic purification systems, and summarized the researches in manipulating intein cleavage reaction. This work is to provide clues for improvement of intein-mediated protein purification.