PKB/Akt regulates the aggregation of actin by Girdin in mouse fertilized eggs.
- Author:
Didi WU
1
;
Panpan ZHANG
2
;
Ying LIU
1
;
Bingzhi YU
1
Author Information
- Publication Type:Journal Article
- Keywords: F-actin; Girdin; PKB/Akt; mouse fertilized eggs
- MeSH: Actins; physiology; Animals; Mice; Microfilament Proteins; physiology; Phosphorylation; Proto-Oncogene Proteins c-akt; physiology; RNA, Small Interfering; Vesicular Transport Proteins; physiology; Zygote
- From: Chinese Journal of Biotechnology 2016;32(9):1204-1211
- CountryChina
- Language:Chinese
- Abstract: The purpose of this study is to reveal the role of Girdin in regulating the aggregation of actin filaments by studying the relationship between PKB/Akt and Girdin. First we used Scansite software (http://scansite.mit.edu) to predict relevant target sites of PKB/Akt on mouse Girdin. To gain insight into the role of phosphorylation of Girdin by PKB/Akt, we assessed the location of phosphorylated Girdin in fertilized eggs by staining with anti-P-Girdin 1 417 Ab. We detected a distinct increase in the fluorescence signal of F-actin and P-Girdin 1 417 after microinjection of Akt WT and myr-Akt. The addition of myr-Akt induced phosphorylation of Girdin in mouse fertilized eggs. In addition, siRNA-mediated Akt-knockdown blocked phosphorylation of Girdin. The distribution of actin filaments was obviously scattered. These results strongly suggest that PKB/Akt could directly phosphorylate Girdin on Ser1 417 and promote its function in mouse fertilized eggs.
